Abstract: 280 bio-bacterial strain of Phytophthora parasitica var.nicotianae was isolated and screened From 141 soil sample, especially inhibition radius of 19 bio-bacterial strain were greater than 17 mms.The greenhouse pot experiment showed that: bio-bacterial strain had control effect on Phytophthora parasitica var.nicotianae respectively, especially HJ01912, the contorl effect is to 73.80 % , but had no no significant diffrence with chemical reagent.