Abstract: 0.5 mg/L 6-BA and 0.5 mg/L NAA are added to the culture based on the last study. KT and 2,4-D concentrations impact on Oxalis triangularis purpurea regeneration system by “one-step method” are studied. It shows that MS + 0.5 mg/L 6-BA + 0.5mg/L NAA + 1.0mg/L KT + 0.1mg/L 2,4-D hormone combination promote the generation of the regeneration system. The callus, adventitious root and bud is induced after certain days. The regeneration system shows high differentiation efficiency, and averagely 21.8 seedlings are induced from 1cm2 leaf explant. “One-step method” induced Oxalis triangularis purpurea regeneration system may reduce the workload effectively, moreover, with a characteristic of high efficiency induced regeneration seedlings, which is propitious to Oxalis triangularis purpurea breed large quantitively.