Abstract: In May 2007, 102 fish of 16 species were collected from 2 sample spots from the Qingdao City and the total mercury concentrations of samples were detected. In this investigation, the result indicated that the range of the total mercury concentrations in fish was 0.049-0.157mg/kg and the average mercury value in fish did not exceed the background value of mercury concentrations in fish in this area and there were no samples whose total mercury value was not in the excess of the standard set by Chinese government. Therefore, the release of total mercury from the sediment in the different kinds of water body is the significant sources for the total mercury contamination in fish in Qingdao City. There was significant difference on the total mercury concentrations in Ctenopharyngodon idellus, Carassius auratusgibeliv, Parasiburusasvtus, Sardina pilchardus collected from region Chengyang and Shinan, while the mercury concentrations in fish, such as Cyprinus carpio Linnaeus, Ooctopus ocellatus, Psendosciaena polyactis, were similar. In all the samples, the trend of the total mercury in fish samples collected from region Chengyang was following: Ooctopus ocellatus > Hypophthalmichthys molitrix > Psendosciaena polyactis> Sardina pilchardus> Cyprinus carpio Linnaeus> Carassius auratusgibeliv > Parasiburusasvtus > Sepiella maindroni > Paralichthysolivaceus > Ctenophar yngodon idellus > Mallotus villosus > Trichiurus haumela > Loligo Chinensis > Aristichthys noyilis > Glyptonotus antarcticus> Sardina pilchardus > Protosalanx hyalocranius > Carassius auratusgibeliv. Moreover, the trend of mercury contents in fish samples collected from region Shinan was following: Psendosciaena polyactis > Ooctopus ocellatus > Ctenopharyngodon idellus > Parasiburusasvtus > Cyprinus carpio Linnaeus. The conclusions from this research pointed that the law for mercury distribution in the different species of fish was: the concentration of mercury in fish in the bottom of the river>that of fish in the surface of the river; and that of predatorial fish>that of herbivorous fish.