Abstract: In order to investigate the effects of water-saving irrigation on stress-resistance physiological factors of rice, the trough cultivation experiment with rain-proof shelter were carried out. The results followed that the relative values of MDA, plasmalemma permeability, soluble sugar and amino acid in leaves increased and then decreased after rewatering to saturated soil under optimal water stress when the soil water potential was higher than -30kPa. The change trend became stable with the advance of growth stage. When the soil water potential was below -30kPa, the relative values of MDA, plasmalemma permeability, soluble sugar and amino acid in leaves significantly increased, increases of MDA and plasmalemma permeability in better drought resistant cultivars were obvious less than those in weaker drought resistant ones, and increases of soluble sugar and amino acid in better drought resistant cultivars were obvious more than those in weaker drought resistant ones, and plasmalemma permeability, soluble sugar and amino acid decreased from16 days to 40days after anthesis. These results suggest that water stress can induce physiological and biochemical changes in rice and changes of MDA content, plasmalemma permeability and osmotic regulators are correlated significantly to drought resistance of rice varieties.