Abstract: According to the nucleic acid sequence from EST database, two specific primers were designed and a 765 bp cDNA fragment containing 3′ untranslated region was amplified by RT-PCR from ripening peach fruit. The cDNA, named PpERF1, contains an ORF (open reading frame) which is 708 bp in length. PpERF1 contains a highly conserved, plant-specific DNA-binding domain, which is composed of three β-sheets and an α-helix. Multiple alignment of the ERF conserved domains shows that PpERF1 has 68.4%-100% identity to ERF homolog in other plants. PpERF1 shared high similarity at both nucleotide and polypeptide level with corresponding sequences from Arabidopsis, grape and pea.