Chinese Agricultural Science Bulletin ›› 2009, Vol. 25 ›› Issue (5): 271-274.
Special Issue: 生物技术
• 热带农业科学 • Previous Articles Next Articles
Received:
Revised:
Online:
Published:
Abstract: Specific primer sequences were designed on the basis of converse motifs of cloned resistance genes and subsequently used as PCR primers to amplify the resistance gene analog(RGA) in banana.The amplified product of BRGA1 was cloned and sequenced.The banana RGA contain some conserved motifs such as p-loop,Kinase-2a,Kinase-3a and HD presenting in known NBS-LRR resistance genes from other plants and share between 23.8%-42.7% amino acid sequence identity with them.The BRGA1 expression was induced and detected by Northern blotting,which is positively regulated by salicylic acid.
0 / / Recommend
Add to citation manager EndNote|Ris|BibTeX
URL: https://www.casb.org.cn/EN/
https://www.casb.org.cn/EN/Y2009/V25/I5/271