Abstract: Abstract: Objective Isolation and Identification of a doubtful enterococcus faecalis strain;Methods through Phenotypic Characteristics containing culture characteristics, Morphological observation, biochemistry experiment etc, combining with the phylogenetic analysis of 16S rDNA; Results The result indicated that this Gram-positive bacteria can grow at 10 ℃ and 45℃,6.5% sodium chloride or pH9.6 also.catalase- negative,can produce acid and no gas from glucose. though phylogenetic analysis of 16S rDNA,we can found that it has topmost homogeneity with E.faecalis;Conclusion These findings may indicated that the isolated strain should be E.faecalis.