Current issue

• 2009 Vol.25 No.10 Published:20 May 2009

  畜牧兽医科学

  Isolation,Identification and Phylogenetic Analysis of a Strain Enterococcus Faecalis Yang Xia, Chen Lu, Chang Hongtao, Liu Hongying, Yang Yingying, Fu Feng, Wang Chuanqing 2009, 25 (10):  1-5.  Abstract + ( 1407 )   PDF ( 604989KB )   ( 1366 ) Abstract: Objective Isolation and Identification of a doubtful enterococcus faecalis strain;Methods through Phenotypic Characteristics containing culture characteristics, Morphological observation, biochemistry experiment etc, combining with the phylogenetic analysis of 16S rDNA; Results The result indicated that this Gram-positive bacteria can grow at 10 ℃ and 45℃,6.5% sodium chloride or pH9.6 also.catalase- negative,can produce acid and no gas from glucose. though phylogenetic analysis of 16S rDNA,we can found that it has topmost homogeneity with E.faecalis;Conclusion These findings may indicated that the isolated strain should be E.faecalis.

  Retrovirus-mediated Expression of Antibacterial Peptide Cecropin B in Mammary Gland of Bovine Wang Tiedong, Pang Daxin, Ouyang Hongsheng 2009, 25 (10):  6-10.  Abstract + ( 1542 )   PDF ( 1059770KB )   ( 731 ) Abstract: We synthesized the code-optimized gene of Cecropin B according to it’s natural sequence by overlap extension PCR, The gene was then cloned into the vector pMD-18T and was confirmed by DNA sequence. In order to get the whole mammary specific expression cassette, the gene of Cecropin B was cloned into the mammary specific expression vector pIRES-Bcp-neo which contain the goat β-casein gene 5'flanking regulation sequence, The whole mammary specific expression cassette was cloned into the retrovirus vector pLNCX, so the retrovirus vector pLNCX-bCP-cecB was constructed, This vector was transfected into a packaging cell line PA317, RNA from the vector is packaged into infectious, replication-incompetent retroviral particles which was then injected directly into the lactating mammary gland of bovines. The expression and bactericidal activity was detected using inhibition zone assay for the bacteria: E.coli K12D31, Antibacterial Peptide Cecropin B expressed in milk possesses bactericidal activity which can last at least thirteen days.

  Preparation of Micro-ecological Feed Additives and Serum of Beef Cattle Physiological and Biochemical Indexes Impact Wang Jinhe, Deng Daijun, Wang Juqiang 2009, 25 (10):  11-14.  Abstract + ( 1607 )   PDF ( 538401KB )   ( 849 ) Abstract: 【Objective】Study and promotion of green scale beef cattle feeding technology， development of Green Growth microecological feed additives,To study their impact on beef cattle serum physiological and biochemical indicators。【Methods】Isolation and screening of the three have bacteria for Preparation of Microecologics，Selection of conditions consistent beef cattle，To study the physiological and biochemical indicators of serum impact。【Results】The experimental results show that，Isolation of bacteria and Preparation of Microecologics to meet the standards, Beef cattle serum hyperthyroidism or the lower three key indicators (FT3, FT4, TSH), in beef cattle before and after the experiment is a change in the normal range, serum growth hormone and insulin-like growth factor Content higher than control group, But the difference was not significant (P> 0.05). 【Conclusion】The results suggest that microecological feed additives for beef cattle serum on the physiological and biochemical indexes without adversely affecting, it is safe to green feed additives。

  Low pathogenic avian influenza virus (LPAIV) H9N2 inoculation into chickens result in secondary infection of Pasteurella multocida Sun Ruiqin, He Hongxuan, Gao Yunying 2009, 25 (10):  15-18.  Abstract + ( 1367 )   PDF ( 599637KB )   ( 750 ) Abstracts: Objective To observe the changes of chickens when post-challenge with LPAIV H9N2. Methods One-month-old SPF chickens were inoculated with 107.4EID50/0.1ml H9N2 viruses, 0.5ml/chick. Results Chickens were sick and a few of them were died. At necropsy, the primary gross lesions in all chickens were severe pulmonary consolidation and multifocal hemorrhage in several organs, including subcutaneous, lung, heart, thymus, stomach, intestine, liver, and lymph nodes; Necrotic foci were in liver, spleen, kidney and lung. The bacterial isolated was identified as Pasteurella multocida based on biochemical characteristics and PCR. Conclusion Low pathogenic avian influenza H9N2 virus may cause secondary infection of Pasteurella multocida, which could results of high rate both morbidity and mortality in chickens.

  农业生物技术科学

  Genetic Diversity of Maize Germplasm from Different Groups by SSR Markers 2009, 25 (10):  22-28.  Abstract + ( 1424 )   PDF ( 872403KB )   ( 801 ) Genetic diversity of 72 maize germplasm resources from 6 groups was studied by SSR marker. 57 primers detected 222 alleles amang the tested materials and per primer detected 2-8 alleles. Nei’s genetic distance varied from 0.0793 to 0.6637. The average genetic distances of the materials within the three special corn groups ( popcorn, sweet corn, waxy corn) were smaller than those amang them significantly. To three normal corns (flint corn, dent corn,semident corn), there were no significant differences of the average genetic distances within the groups or amang the groups. Variation range of genetic distance amang the special corn groups was smaller than amang the normal corn groups and between the two types also. Based on cluster analysis by UPGMA, 18 special corn inbred lines could be divided into 3 groups and coinsided with their group classification. Most of tested normal corns could be divided into 5 heterotic groups and mainly belonged to PB(31.5%), Lüda Red Cob(16.7%), and BSSS(14.8%).

  Genetic diversity and differentiation among four populations of Flaveria bidentis in Hebei provinc, China 2009, 25 (10):  29-35.  Abstract + ( 1459 )   PDF ( 1853431KB )   ( 1104 ) AFLP was used to analyze genetic diversity and differentiation of four populations of Flaveria bidentis(L.)Kuntze in Heibei province,chian; Information about four populations was also investigated. The results showing relatively high genetic diversity and significant genetic differentiations among four populations of F.bidentis(L.)Kuntze. The results of analysis indicating genetic diversity increased rapidiy accompanied by time and expanding of the population scale;the population in similar latitude of its origin had the highest genetic diversity.the formation of Genetic structure at present it is because its characteristics of life history and invasion ecology, and the stage of population. Seedsof F. bidentis are spread by w ind and water resulting in close biogeographic relationships among the populations occurring in neighboring regions

  The relations between the blade anatomical structure and characters of tea varieties in Guangxi Qin Xiuju, Li Fengying, He Jiandong, Liao Shaomao, Deng Huiqun, Luo Xiaomei, Peng Youbing 2009, 25 (10):  36-39.  Abstract + ( 1484 )   PDF ( 727533KB )   ( 848 ) This papers explore the correlation between the thickness of blade、cuticula、epidermis cells、fence form、sponge form，the ratio of the fence form and sponge form，the layer of the fence form，the length of cell and the yield，suitable system，resistance of the blade anatomical structure by paraffin section method and observing in optical microscope using tea varieties in Guangxi，such as the No.1 of Guilv， and provide a scientific basis for tea scientific research and production。

  AFLP Molecular Marker and Its Application in Dry Fruit Bai Ruixia, Peng Jianying, Zhang Yuan, Li Li 2009, 25 (10):  40-46.  Abstract + ( 1357 )   PDF ( 561531KB )   ( 804 ) AFLP (Amplified fragment length polymorphism) is a highly efficient technique for DNA fingerprinting. The technique is applicable to any organism without prior sequence characterization of the target genome. It’s characterized with reliability of RFLP, as well as with simplify of RAPD. In recent years, AFLP markers have been widely applied to dry fruit genetics and breeding. In this paper, the basic principles and procedures were introduced. The application of AFLP in dry fruit germplasm resource research, genetic linkage map construction, gene location, molecular-assisted selection and gene expression were reviewed. Problems and prospects of AFLP application in dry fruit genetics and breeding were also briefly discussed.

  Progress in Genetic Engineering Improving Vitamin E Contents in Oilseeds FAN Xing-Fu , Song Shu-Min , Tian Bao-Ming , LI Xu-jiao , SHI Gong-yao , PEI Zhen-qiang , WANG Bo-nan , GAO Shu-guang 2009, 25 (10):  47-52.  Abstract + ( 1610 )   PDF ( 594976KB )   ( 991 ) Vitamin E, the indispensable nutritive value of which has been revealed, was originally discovered as an essential element for reproduction. Vitamin E has great ability of antioxidation and health protection. Edible plant oil is particularly rich in vitamin E. As an effective oxidant, vitamin E plays an important role in protecting oil from being oxidated by free radicals and from rancidity, which could enormously prolong its shelf life. The past large number of researches on vitamin E metabolism have revealed all the steps in vitamin E biosynthesis, making it possible to regulate vitamin E content through genetic engineering. To date, researches to improve vitamin content in oilseed crops have been mainly focused on the tow following strategies, enhancing the total volume of vitamin E and increasing the percentage of α-tocopherol, the form of vitamin E displaying the greatest efficacy.

  Researches and application on Molecular Markers of Powdery Mildew Resistant Genes in Wheat 2009, 25 (10):  53-58.  Abstract + ( 1588 )   PDF ( 491409KB )   ( 1352 ) The use of resistant cultivars is an effective, economical, and environmentally safe approach that eliminates the use of fungicides and reduces production losses due to powdery mildew caused by Blumeria graminis f. sp. tritici (syn. Erysiphe graminis f. sp. tritici). So far, 55 genes/alleles for resistance to powdery mildew at 39 loci have been identified and located on 16 different chromosomes,of which 30 resistance genes/alleles have been tagged by different molecular marker systems. Several quantitative trait loci (QTLs) for adult plant resistance to powdery mildew have been associated with molecular markers. The detailed information on chromosomal location,the sources, available molecular markers and cloning of these genes has been reviewed. The fresh researches, existed problem and the resolvent in marker-assisted selection using these genes are also discussed.

  The genetic difference of Flinor and Mingxian169 analysed by microsatell ite marker 2009, 25 (10):  59-62.  Abstract + ( 1484 )   PDF ( 734843KB )   ( 757 ) Simple sequence repeat (SSR) molecular marker was used to analyze the frequency of polymorphism of wheat varieties – Flinor and Mingxian169. The result was followed. Of 1243 SSR markers used to investigate DNA polymorphism of the two cultivars, 383 markers could express the polymorphic and cover 31.10%, 127 markers had no clear band, and 733 markers had no polymorphic and covered 59.35%. The polymorphism of BARC, WMS and CFA was higher with 34.98%, 34.72 and 36.49, respectively, while the polymorphism of CWEM was lower with 8.89%. The distribution frequency of polymorphic markers was different among the chromosome in the A, B and D genome, the highest in A genome (41%), lower in B genome (35%) and the lowest in D genome (24%).

  Analyses of germplasm by RAPD in Ardisia crenata Sims. DENG Su-Fang , , 2009, 25 (10):  63-67.  Abstract + ( 1424 )   PDF ( 1026889KB )   ( 891 ) 15 accessions from 3 kinds of germplasm were used to analyse the genetic diversity and to carry out cluster analysis by means of RAPD in Ardisia crenata Sims. 17 random primers, which were selected out of 200 random primers, generated 247 bands, and 92.71% of them were polymorphic. The result of cluster analysis showed that there were significant differences among the 3 kinds, which accorded with the result obtained from morphological classification.

  A preliminary study of regeneration system of winter wheat immature embryo and its Agrobacterium transformation ZHANG Bin , , , , 2009, 25 (10):  68-71.  Abstract + ( 1444 )   PDF ( 1924965KB )   ( 682 ) Abstract: Immature embryos of a common wheat variety Linyou145 was used as explant to induce regeneration and transformation,respectively. The immature embryo were inoculated on the MS medium with 2 mg·L-1 2,4-dichlorophenoxyacetic acid to initiate calli. Then,they were inoculated on the MS medium with 1mg·L-1 ZT and 1mg·L-1 IAA, cultured for 2-3weeks for plant differentiation.Finally,the calli of differentiation were transferred into the MS medium with 0.5mg·L-1 NAA for plant regeneration.Following the above protocol,987calli,123resistant callus and 34 plantlets were obtained from 1229 embryosintotal, Furthermore, plasmid DNA of pCAMBI1301 containing GUS gene as the donor of alien gene was transformed into the immature embryo calli by Agrobacterium tumefaciens.By means of PCR amplification, hygomycin resistant plantlets were obtained. The result from X-gluc staining showed that GUS gene gave transient expression successfully in the transformed calli.Blue spots were observed in resistant calli.

  Advances in Tissue Clutrue of Gerbera jamesonii 2009, 25 (10):  72-76.  Abstract + ( 1515 )   PDF ( 558577KB )   ( 898 ) Advanced in tissue culture of Gerbera jamesonii in past few years are reviewed，which in cludes expants selection,inducing differentiation and transplantation of test tube Plantlet．Summarized the Primary problems in tissue culture of Gerbera jamesonii at present；And the effects of Vc and Transgenic Explant on it.Put forward the problems deserving further research are put forward．

  Comparison and Improvement of Different Extracting Total RNA Methods from Stigmas in E.Sativa Mill. 2009, 25 (10):  81-83.  Abstract + ( 1425 )   PDF ( 641590KB )   ( 713 ) On the basis of guanidine thiocyanate method, an economical method of extracting and puring total RNA from Yunjie stigmas was established.The results showed that the total RNA were in good integrity and high purity, which demonstrated that RNA samples were free of contamination of protein,DNA,polysaccharide and polyphenol ,and were applicable to cDNA synthesis,DDRT-PCR,northern blotting and so on.This method was simple,economic, and of stable performance, good repeatability as well as suitable for extracting total RNA from many materials.

  食品科学

  Research of Pyrolin on Strawberry Preservation Wei Yueqin, Ai Qijun, Wu Zhenyu 2009, 25 (10):  84-88.  Abstract + ( 1326 )   PDF ( 792302KB )   ( 772 ) Abstract: Quality of strawberry fruits coating with pyrolin of different concentration and storied at ambient (25℃) and cold temperature (4℃) respectively were investigated. The results showed that the treatment of coating with pyrolin can markedly reduce the ratio of the losing weight, the loss of flesh firmness and the loss of titratable acid content of strawberry compared to control, and the shelf life were prolonged. The fruits of strewberry coating with 0.313mg/ml pyrolin has the most effective preservation.

  农业基础科学

  The response of three maize inbred seedling with the different nitrogen use efficiency to light and nitrogen , , , 2009, 25 (10):  89-95.  Abstract + ( 1570 )   PDF ( 653936KB )   ( 832 ) Based on the hydroponic culture methods, the responses of the biomass, shoot/root ratio, the nitrate reductase activity and glutamine synthetase activity of 3 maize inbred seedlings with different nitrogen use efficiency to 3 levels of light intensity and 3 levels of N supply were studied. The results showed that the difference of the response of the seedling biomass, shoot/root ratio, the nitrate reductase activity and glutamine synthetase activity among 3 maize inbreds was significant. The biomass of maize seedling with lower nitrogen use efficiency was higher than that of maize seedling with higher nitrogen use efficiency under low nitrogen and light intensity condition, but it was lower under high nitrogen and light intensity condition. The root growth of maize seedling with higher nitrogen use efficiency was better that of maize seedling with higher nitrogen use efficiency. The best combination of nitrogen and light intensity for the biomass and nitrate reductase activity were different among 3 maize inbreds with different nitrogen use efficiency and the maize inbrd with low nitrogen use efficiency need less nitrogen or lower light intensity than the maize inbred with high nitrogen use efficiency. In conclusion, the appearance of nitrogen use efficiency for maize inbreds is not only related to the nitrogen supply but also to the light intensity.

  Research Progress on Nitrate Reductase functions in Plants , , 2009, 25 (10):  96-99.  Abstract + ( 1565 )   PDF ( 437636KB )   ( 1752 ) Nitrate Reductase (NR) is a key enzyme for nitrate assimilation in plants. Through its catalytic reaction, it not only regulates nitrate reduction but also influences the carbon metabolism of photosynthcsis. Exploring additional physiological functions of NR. and understanding the functioning mechanisms will be of great significance both in theory and practice. The rapid developments of molecular biology makes it possible to understand the NR's physiological functions and the underlying mechanisms at the molecular level. Further, through the molecular approaches we may be able to know the relationship between nitrogen metabolism and other metabolic pathways, and also relationship between NR, NO and abiotic or biotic stresses. Research prospects on nitrate reductase.

  Research progress of creating and testing technology on maize mutants 2009, 25 (10):  100-103.  Abstract + ( 1580 )   PDF ( 492925KB )   ( 1179 ) It is very difficult to create maize mutants , and even more difficult to detect useful mutants and clone related genes . This paper summarizes the latest research progress in the creation and detection methods of maize mutants, and intends to explore more effective ways of creating and detecting technology on maize mutants.

  Study on Karyotype of Anemarrhena asphodeloides Bge. 2009, 25 (10):  104-106.  Abstract + ( 1431 )   PDF ( 688980KB )   ( 733 ) The karyotype of Anemarrhena asphodeloides Bge. was studied. The results show that the chromosome number was 2n=22 and its karyotype formula is K(2n)=2x=22=16m+6sm. The karyotype of Anemarrhena aphodeloides Bge. belonged to “2B” Stebbins.

  林业科学

  The analysis for the pine bark scale ,Matsucoccus matsumurae (Kuwana) invasion and spread possible in the Northeast China Wang Zhiming, Du Jisheng, Zhang Lijun, Zhong Xiaowei, Pi Zhongqing 2009, 25 (10):  107-111.  Abstract + ( 1226 )   PDF ( 1127195KB )   ( 669 ) According to the nature geography condition and the forest stand distribution situation which probable be harm and parasitism by the pine bark scale Matsucoccus matsumurae (Kuwana), the article analysis its invasion history, its adaptation to the cold dry-climate and the possibility of further spread of routes, and forecasts the scale spreading ways and means in Jilin and Heilongjiang, this warns people should be pay great attention to the scale.

  Improving Germination of Elaeagnis mollis Diels Seed by Low Temperature and PEG Pretreatment 2009, 25 (10):  112-115.  Abstract + ( 1361 )   PDF ( 555290KB )   ( 939 ) The effects of low temperature and PEG pretreatment on germination of E. mollis Diels Seeds were investigated. And we found that the infiltration effect was evident when pretreated with cood stress at 2℃ and 25%PEG. The seed vigor index and germination velocity was improved remarkably. The seedling quality and Stress Resistence were improved too. the physiological and biochemical changes of the seeds were examined. Under the treatment conditions, the content of soluble Proteins，boiling-stable Proteins，as well as the activity of superoxide dismutase(SOD). and catalase(CAT) increased while the MDA content and the activity of peroxidase (POD) decreased.

  Tissue Culture And Rapid Propagation Of Red Toona sinensis Zhou Yuling, Yu Huilin, Liu Guangqing, Li Yanping, Zhang Fujuan, Jiang Shuguang, Sun Fengling 2009, 25 (10):  116-119.  Abstract + ( 1745 )   PDF ( 1218409KB )   ( 784 ) Abstract： We sifted out suitable medium of rooting and induced budding by adopting methods on experiment with four-factor three-level orthogonal design using stems of Red Toona Sinensis as explants for plant tissue culture.We also sifted out the suitable medium for rooting of the seedings from differentiation of stem of Red Toona Sinensis I acording to the rooting number in different medium and sifted out the suitable medium for budding of thicket from differentiation of stem of Red Toona Sinensis I acording to the rooting number in different medium, so that we can grip the suitable ratio among the different auxins. The results show that: the medium of （MS+6-BA0.1mg/L+NAA1.0mg/L+IBA1.0mg/L+ZT0.2mg/L） is optimal for rooting in the nine mediums and the medium of MS+BA 0.1 mg/L+NAA 0.1～0.2 mg/L is the best one of proliferation medium.

  农艺科学

  Studies on The Characteristics of C-N Metabolism in Leaf and Starch Accumulating in the root tubers of Different Cassava Cultivars Lu Feiwu, Luo Xinglu, Li Hongyu, Mo Fan, He Yuanlan 2009, 25 (10):  120-124.  Abstract + ( 1790 )   PDF ( 709805KB )   ( 1012 ) Abstract : C-N Metabolic Characteristics in Leaf and Starch Accumulating Developments in the root tubers of cassava during in Cultivars with Different starch content were studied with 3 cassava varieties(SC201,GR891, nanzhi199)in this experiment. The results showed that from the root tuber setting stages to the ripping stage,it had an positisive correlation between Starch content of tuberous root, GS activity of leef ,SPS activity of leef, SS activity of leef and Total soluble sugars content of leef，but it had an significant and positisive correlation with SS activity of leef. And it had an negative correlation with NR activity of leef. But it showed an high opposite negative correlation between Sucrose content of leef, Total soluble sugars content of leef and Amino acid content of leef during the same time. It showed that“Source”had strong supplying ability of assimilating matter in high starch content ones. Three cassava varieties had the same discipline.

  The Study on the Heterosis of New Type Tobacco Hybrid Generation , , 2009, 25 (10):  125-131.  Abstract + ( 1356 )   PDF ( 528845KB )   ( 800 ) In order to study the heterosis of tobacco hybrid F1 generation, 8 materials of novel tobacco Cultivars, which include Zisu tobacco, Mantuoluo tobacco, Luole tobacco, MSG-28 tobacco, G-28 tobacco, M.Zisu tobacco, M.Mantuoluo tobacco, M.Luole tobacco, are recorded by observing their duration period, main agronomic characteristics, and their production quality and quantity situation. Through analyzing those data, it shows: those F1 generations do have the strong heterosis in the seedling stage. However, during other stages of growing in fields, there is no outstanding superiority. The reason why the superiority is not obvious during the later period may caused by the external environment.

  Influence of Different Density on Yield and Quality of Zhenghuangnuo No.2 zhoubo , , , 2009, 25 (10):  132-136.  Abstract + ( 1626 )   PDF ( 572557KB )   ( 812 ) 2008年在郑州市做了郑黄糯2号密度试验，通过对生育期、植株性状、穗部性状和子粒品质分析比较，结果表明：密度对郑黄糯2号产量和子粒品质影响显著。随着种植密度的增加，郑黄糯2号产量先升后降，郑黄糯2号蛋白含量先上升，然后趋于平稳，油分含量呈下降的趋势，淀粉含量变化无规律。为该品种大面积生产提供科学依据。

  Genetic analysis of agronomic traits of hybrid F1 from different wheat cultivars and between wheat and triticale Fan Cunhu,, Wang Shuguang, Sun Daizhen 2009, 25 (10):  137-139.  Abstract + ( 1480 )   PDF ( 402748KB )   ( 678 ) Abstract: To provide the basis for breeding wheat varieties with good qualities using hybridization method, the paper analyzed the genetic laws of hybrid F1between 9 wheat varieties and Jinnong 190 , as well as interspecific hybrid F1 between 7 triticale varieties and Jinnong 190. The results showed that most agronomic traits of hybrid F1 are quantitative, and super-parents or between the two parents, except for awn . The ash green leaf and stem , long spike and red unfull grain from triticale are expressed in interspecific hybrid F1, which are regard as typical traits of identifying hybrids.

  Study on the Effect of Difference Temperature to Soybean Seed Germination Chen Lijun, Guo Qiang, Liu Yingxue, Li Xiangyu, Zhao Yuanling, Dan Dapeng, Chen Qingshan 2009, 25 (10):  140-142.  Abstract + ( 1671 )   PDF ( 434016KB )   ( 1130 ) Through the study of the effect on using the six different temperature : 4℃、8℃、12℃、15℃、20℃、25℃ for Donong42, Dongnong43, Dongnong163, Hefen25, and Suinong10 ,the results showed that soybean seed couldn’t germination at the 4℃ and 8℃. The temperature of highest percent germination was 12℃, which was difference from other’s study. At the same temperature, the percent germination and the average length of sprout was varied significantly in the 5 varieties. To the same variety, the longest sprout’s length and the average length of sprouts increased when the temperature went up. Experimental research was conducive to soybean fields to determine seeding temperature, for timely early seeding, to ensure that field seed germination, emergence, seedling and timely provide the basis for mature and lay the foundation for the ultimate high-yield.

  植物生理科学

  Study on diurnal variation of photosynthetic physiological characteristics of Cornus wilsoniana clones during the flowering stage Song Qing’an,,, Li Zhihui, Li Changzhu, 2009, 25 (10):  143-147.  Abstract + ( 1386 )   PDF ( 563651KB )   ( 654 ) In order to explore the diurnal variation of photosynthetic physiological characteristics, the diurnal variation of photosynthesis of different Cornus wilsoniana clones were conducted using the Li-6400 portable photosynthetic system during the flowering stage. The results showed as following: (1) The diurnal variation of net photosynthetic rate (Pn) of G-2、G-3、G-8、G-9 showed double-peak curve, while G-4、G-5、G-6、G-7、G-10、G-11、G-13、G-14、G-15 revealed single-peak one. The daily average of Pn of 12 clones could be divided into 3 categories. It is, G-6、G-7、G-8 for the first category with the maximum daily average of Pn, G-2、G-3、G-4、G-9、G-10、G-11、G-13、G-14、G-15 for the second category with the medium one, and G-5 for the third category with the minimum. (2) Similarly, based on the daily average of Tr, all the 12 clones could be diveded into 3 types too, namely, G-5、G-14、G-15 for low water consumption types, G-13 for medium one, and G-2、G-3、G-6、G-7、G-8、G-9、G-10、G-11 for the highest one. (3) G-8、G-14、G-15 belonged to high water use efficiency types, G-6、G-7、G-11、G-13 to medium one, and G-2、G-3、G-5、G-9、G-10 to low one. The WUE showed a fluctuating tendency, G-5、G-6、G-7、G-8、G-15 belonged to fluctuating clones, while G-9、G-10、G-11、G-13、G-14 showed more stable, and G-2、G-3 were the most stable ones.

  Effects Of Different Plant Hormone Concentrations On StemTissue Culture Of Caragan microphylla 2009, 25 (10):  148-150.  Abstract + ( 1370 )   PDF ( 445467KB )   ( 700 ) Stalk segments of Caragan microphylla Lam were cultured as explants on medium supplemented with plant hormone in different concentrations.The results showed that Murashige and Skoog(MS) medium supplemented with NAA 1.5mg/L,6-BA0.5mg/L was the most effective medium for the differentiation and multiplication of the shoots. The induction rate was 87.3%. The optimum rooting medium was MS+IAA5mg/L.

  土壤肥料科学

  Effect Research of Potassium Fertilizer and Density to Huaibei area Cotton Yield and Quality Wu Lanyun, Li Huang, Liu Xian, Li Jianhua, Xu Maolin, Zhou Debao 2009, 25 (10):  151-155.  Abstract + ( 1391 )   PDF ( 620731KB )   ( 666 ) In order to study the density of potassium and Huaibei area of cotton yield and quality of 2006-2007, we made two years in a row of cotton cultivation test using the second saturated D-optimal design, to explore the density and fertilizer on cotton The production, and fiber length of the rupture of the value of the effect of the combined effect of the establishment of the equation.The results：The density of potash fertilizer and seed cotton lint production and both are related ，K and density of seed cotton and lint against each other is also a positive effect， Lint and seed cotton production are the highest range of facilities potassium 400.2 kg/hm2--409.0 kg/hm2, the scope of planting density is 36,136 / hm2 - 37973 strain / hm2; Cotton fiber length and fracture values reflect the effects of equation, potash fertilizer and density of the two types of quality indicators are positive effect, cashmere long the effect of potash fertilizer is greater than the density, fracture density effect is greater than the value of potash; fiber length highest Shi Potassium is the scope of 315.5 kg/hm2--367.7 kg/hm2, the density of 25,569 / hm2 - 26229 strain / hm2。

  Effects of nitrogen application combined with potassium on yield and quality of strong gluten wheat 'Jimai 20' zhaochangxing , , , , 2009, 25 (10):  156-160.  Abstract + ( 1282 )   PDF ( 508681KB )   ( 574 ) Abstract: In order to provide theoretical basis and technological for high-quality and high-yield cultivation of a strong gluten wheat variety,Jimai 20,the experiment studied effects of the radio of nitrogenous fertilizer and potassic fertilizer on yield and qualitly of Jimai 20 under the field conditions with High-yield cultivation。The results showed that the effects of nitrogen and potassium and their interaction on wheat grain yield and its component factors had reached significant level or very significant level,and the handling of N 225 kg、K2O 150kg per hectare had the highest yield. With the increasing application of nitrogen and potassium , flour yideld and bulk density showed the trend of first increase then decrease, grain protein content and wet gluten content, the sedimentation value increased with the nitrogen . In the range of application K2O 0 to 150 Kg per hectare ,Grain protein content and wet gluten content increased with the potassium, When reached to 210 per hectare they decreased,and sedimentation value decreased with the potassium. The combined application of N and K haven’t significant effects on grain protein content, but the effects have achieved significant levels on wet gluten content and sedimentation value. Nitrogen fertilizer can increase the elasticof wheat dough, potassium fertilizer can improve the doughy extensibility,and the combined application of N and K can significantly increase the extension area.

  Study on Water and fertilizer retaining effects of Anti-drought fertilizer yun jiang , , , , , , 2009, 25 (10):  161-165.  Abstract + ( 1515 )   PDF ( 490208KB )   ( 741 ) Anti-drought fertilizer (ADF)can be made by adding some kinds of Organic and inorganic mineral and chemical fertilizers into super absorbent polymer by special techniques．The pot experiment on Maize was carried out to investigate water—retaining and fertilizer use effects of ADF under water-controlled conditions．The result showed that the ADF applied could improve the Maize plant yield significantly, water use efficiency and N element use efficiency .Compared to applying ordinary fertilizer，the Maize plant yield was increased by 14.8-27.2%,48.4%-68.8% in the water use efficiency, 24.5%-45.2% in N element use efficiency by applying anti-drought fertilizers.

  The Research of the High Production Applies Fertilizer TechnicalPattern of Summer Peanut On Yudong Plain Yang Jiyuan, Zhang Shenju, Hou Yuexin 2009, 25 (10):  166-169.  Abstract + ( 1551 )   PDF ( 541758KB )   ( 736 ) 采用5因素二次回归正交旋转组合设计的试验方法，研究了夏花生产量与有机肥、氮肥、磷肥、钾肥和石膏施用量的关系及其5项施肥因素之间的交互作用，建立了产量目标性状技术模式。通过模拟寻优，筛选出了6000㎏/h㎡以上夏花生荚果产量的优化施肥方案：有机肥62115.0～65745.0 kg/hm2，氮肥（N）219.2～237.9 kg/hm2，磷肥（P2O5）133.1～144.7 kg/hm2，钾肥（K2O）57.8～75.6 kg/hm2，石膏（CaSO4）186.8～202.7 kg/hm2。

  Effects of wheat/oat different root barriers and Mn fertilizer in soil on Mn nutrition of wheat ZHAO ZHAO Xiu-Fen , , 2009, 25 (10):  170-173.  Abstract + ( 1304 )   PDF ( 470295KB )   ( 728 ) A root box experiment was conducted to investigate effects of different root barriers and Mn fertilizer in soil on Mn nutrition of wheat in wheat/oat intercropping system. Results show that application Mn feitilizer in soil led to significantly higher shoot dry weight and Mn uptake of wheat compared with the check. Shoot dry weight, Mn concentration and Mn uptake of wheat were no obviously effect by wheat/oat different root barriers. The abilities which uptake and activation soil Mn of different crop cultivars was different, namely oat＞wheat, 9023＞Chuanmai28, Bayou4＞Bayou3. But Mn nutrition of wheat was not improved by intercropping with oat, the possible reason was plentiful plants leading to lacking space of root growth, the essential reason will be farther research. Key words wheat/oat; root barriers; Mn nutrition

  Effects of Pretreatment on content of soil NO3-N、NH4-N zhao ruifen , , , 2009, 25 (10):  174-177.  Abstract + ( 2208 )   PDF ( 474654KB )   ( 1127 ) Extracting methods of soil NO3-N、NH4-N by KCl solution and determining that by continuous flow analyzer were respectively used. There were many factors to influence results of soil NO3-N、NH4-N, such as soil pretreatment, concentration of KCl extracting solution and ratio of solution to soil and so on . In the paper , effects of these factors on content of soil NO3-N、NH4-N in greenhouse soil were studied, The results showed that : concentration of soil NO3-N、NH4-N in greenhouse had significant changes during air-drying and heat-drying, and contents of NO3-N、NH4-N in fresh soils was lower than air-dried soils and heat-dried soils. When 2M KCl extracting solution was used and the ratio of solution to soil is 5:1, determined results of soil NO3-N、NH4-N in greenhouse were more accurate. but fit extractant concentration and ratio of solution to soil must be selected according to the goal of the research .

  园艺园林科学

  Gene Expression of Chilling Tolerance Induced by Salicylic Acid in Cucumber Seedling 2009, 25 (10):  178-182.  Abstract + ( 1527 )   PDF ( 748138KB )   ( 757 ) Abstract ：【OBJECTIVE】Salicylic acid (SA) was found to improve biotic and abiotic tolerance of plants. In present paper cucumber “Xinyou30” was employed to study the effect of SA on plant gene expression and its mechanism. 【METHOD】Under chilling stress at 7℃ cucumber seedlings were sprayed by distilled H2O and SA (3mmol/L, pH6.0), respectively. After 48 hours total RNA was extracted from cucumber leaves and reversely transcripted into first strand cDNA. mRNA differential display was applied to analyzed SA-induced cDNA in cucumber leaves. 【RESULTS】Twelve SA-induced cDNA fragments were isolated and retracted. Two （SICCL_1 and SICCL_2）of them were affirmed by reverse dot blot to be significantly induced by SA. Nucleotide sequence analysis showed that SICCL_1 had 88% identity to AM734282 related with Cucumber Mosaic Virus (CMV ) in Cucumis melo subsp. melo; SICCL_2 had86% identity to THL24h-106 related with NaHCO3 stress in Tamarix hispida. 【CONCLUSION】It is postulated that SA may improve tolerance of plants via inducing overlapping genes against biotic and abiotic stresses.

  ‘Fengdanbai’ Embryo culture and Plantlet Regeneration of peony 2009, 25 (10):  183-186.  Abstract + ( 1385 )   PDF ( 1020665KB )   ( 742 ) Abstract: ‘Fengdan’embryos were used as initial esxplants, the effect of the following factors on induction callus and differentiation adventitious buds:basal medium,plant hormone and stacked volume time were studied .Results indicated that the optimal time for embryos germination was 40d; the optimal medium for embryos induction callus was ＭＳ+Ca2++2 mg•L-1 2,4-D+30ｇ•L-1sucrose,the induction frequency was 85%;The optimal medum for differentiation adventitious buds was MS+ Ca2++1.0 mg•L-16-BA+0.5 mg•L-12,4-D+20ｇ•L-1.sucrose.Shoots with 1.5cm high were transferred to rooting medium and rooting frequency reached 80%.

  Effect of tow rootstock on aroma components on‘Brookes’ sweet cherry 2009, 25 (10):  187-190.  Abstract + ( 1915 )   PDF ( 505931KB )   ( 932 ) Abstract:【OBJECTIVE】In order to study the effects of rootstock on aroma components of sweet cherry.【METHOD】The headspace solid-phase micro-extraction coupled with capillarygas chromatography- massspectrometry (GC-MS) was used for the determination of volatiles in harvested ripe sweet cherry of Brookes fruit（Prunus.avium L.）.【RESULTS】A total 61 compounds were identified from the samples , alcohols, aldehydes, and esters were the major constituents. 38compounds were identified in Brookes(Jisaila root stock), representing 59.66% of the total peak area; The main contents were Alcohol , Hexyl acetate , Styrene , 1-ethyl -3- methyl –Benzene , 1,2,3-Trimethyl benzene. 30compounds were identified in Brookes(Colt root stock), representting 64.74% of the total peak area.;The main contents were Alcohol, (E)-2- Hexen-1-ol,3-t-Butyl-oct-6-en-1-ol,Hexyl acetate.【CONCLUSION】The rootstocks influence to be obvious on aroma components of sweet cherry, Jisaila root stock has the obvious superiority. (E)-2- Hexen-1-ol, Hexanal, Hexyl acetate, Butanoic acid, 2-hexenyl ester, (E), 3-t-Butyl-oct-6-en-1-ol were the characteristic aroma components of‘Brookes’sweet cherry fruit.

  植物保护科学

  Ustilago crameri koern physiological race Preliminary Research 2009, 25 (10):  191-196.  Abstract + ( 1247 )   PDF ( 562531KB )   ( 633 ) Ustilago crameri koern in our province is one of the major diseases in recent years, there are signs of increase. The test adopted by the different varieties of resistance and cross-species around the vaccination, the identification of pathogenic changes in the analysis of Ustilago crameri koern division race. The results show that are at least three types of physiological race, that are, Xinzhou and Huguan low pathogenic two types , and another one highly pathogenic. Most of the major diseases by this rice. It is our province millet production potential threat.

  A Separation Appraisal on the Root-Knot Nematode in the Clerodendranthus Spicatus.(Thunb.)C.Y.Wu and the Influence Which Grows to the Clerodendranthus Spicatus. Yu Xu-Dong , , , , 2009, 25 (10):  197-201.  Abstract + ( 1427 )   PDF ( 2702529KB )   ( 641 ) Abstract: [Objective] Understand the type and characteristic of the Root-Knot Nematode in the clerodendranthus spicatus.(Thunb.)C.Y.Wu and the influence which grows to the clerodendranthus spicatus.[Method] Separates the Root-Knot Nematode’s female and male and larva from root system which the clerodendranthus spicatus. were infected with the Root-Knot Nematode, view and describe the Root-Knot Nematode’s characteristic under a microscope and discern the type through compares the Root-Knot Nematode’s characteristic pattern. Study the influence of growth which the clerodendranthus spicatus. were infected through the pathogenic experiment, and make an objective appraisa through viewing the clerodendranthus spicatus.’s growing trend and setting-out content of the chlorophyll and protein.[Result] Separated and get the clerodendranthus spicatus. (Thunb.)C.Y.Wu.’s Root-Knot Nematode, and has succeeded in doing the pathogenic experiment .[Conclusion] The Root-Knot Nematode in the clerodendranthus spicatus.(Thunb.)C.Y.Wu is the Meloidyne incognita. .It is serious interfere with the clerodendranthus spicatus.’s growth. The clerodendranthus spicatus. is doing bad even died, the important metabolism material like protein and chlorophyll’s content is obvious drops.

  Studies of risk assessment of Hyphantria cunea (Drury) in China Li Shuxian, Gao Baojia, , Zhang Dongfeng, Ning Chao, Qu Jinliang 2009, 25 (10):  202-206.  Abstract + ( 1549 )   PDF ( 644707KB )   ( 794 ) Abstract：To assess the risk of the invasive pest Hyphantria cunea(Drury), to know its potential distribution and the ratio of occurrence, make great sense for monitoring and controlling. Using GARP (Genetic Algorithm for Rule-set Prediction Modeling ), The article predicted suitable distribution area of Hyphantria cunea (Drury) in China, And the risk of Hyphantria cunea (Drury) in part area of Hebei province was assessed with fuzzy method based on information diffusion principle. the result showed that the values of risk decreased with the increasing of disaster index levels. the potential suitable distribution is from 21.20°N～46.33°N, 97.80°E～132.11°E and 36.81°N～41.85°N, 76.00°E～94.66°E (part areas of Xinjiang). Based on GARP and fuzzy method based on information diffusion principle, assessed the risk of the invasive pest using known distributing data, offering gist for monitoring, controlling and forecasting, have abroad application foreground.

  Effects of Different Tillage Methods on Weed Dynamics and Yield in Corn Field FAN Cui-Qin , , , 2009, 25 (10):  207-211.  Abstract + ( 1599 )   PDF ( 542812KB )   ( 877 ) Abstract: Field experiment was conducted to study effects of different tillage methods on weed dynamics and weeds control efficiency of nicosulfuron spraying in 2-4 leaf stage of weed and yield of corn in wheat-maize double cropping system. The results showed that weed communities had little different in different tillage methods. The numbers of weed reduced 53%-82% comparing with the plot of wheat stalk-covering and no tillage, and reduced 62%-78% comparing with the plot of wheat stalk-covering and rotary tillage. At the rate of 30g/hm2 of nicosulfuron weeds control efficiency in wheat stalk-covering was the best in three tillage methods. The emergence percentage of corn were lower in wheat stalk-covering than that in no tillage and rotary tillage, but it’s seeds per ear , 100-seeds weight and the yield of corn were higher. At the rate of 45-60g/hm2, the yield of corn had little different in different tillage methods. At the rate of 30g/hm2 of nicosulfuron and CK, the yield of corn in wheat stalk-covering was the highest in different tillage methods.

  Study on Resistance and Control Technology of Strawberry Powdery Mildew in Greenhouses Zhou Zengqiang, Hou Hui, Wang Li, Feng Guixin, Zhou Houcheng 2009, 25 (10):  212-214.  Abstract + ( 1321 )   PDF ( 404482KB )   ( 852 ) Study on occurrence period and variety natural disease resistance of strawberry powdery mildew in greenhouses, The results indicate that strawberry powdery mildew occurred is general on leaf in February to March, disease becomes aggravated in April, and the highest could reach in May. Results also showed that disease occurrence was milder on fruit before March, and disease condition becomes more serious after April. The high resistance, resistant, susceptible, highly susceptible were 8, 5, 3 and 3 of 19 strawberry varieties, there was obvious resistance difference between cultivars. Study on screening out fungicides proved that the effect of nine fungicides such as difenoconazole and hexaconazole was above 80%, had a better effect on strawberry powdery mildew.

  生态农业科学

  Ecological Footprint Of Fuzhou Based On The Emergy Theory 2009, 25 (10):  215-219.  Abstract + ( 1393 )   PDF ( 565691KB )   ( 669 ) Ecological footprint is a method for measure sustainable development degree of a country or region, quantitatively reflect the destructiveness and influence degree of human activity on ecological environment. Based on simple explanation of conventional modle of ecological footprint, put forward a modified modle by combining EMA with traditional ecological footprint of calculation, analysis the ecological footprint of Fuzhou city in 2006. The results show that this year, the average ecological footprint of Fuzhou was 1.29 hm2, the average ecological carrying capacity was 0.17 hm2, and the ecological deficit was 1.12 hm2,Fuzhou was unsustainable at present, which conforms to the result of conventional method. But the modified modle got more reasonable factors and give a more realistic picture of resource utilization situation and sustainable development ability, compared to the conventional one.

  农业资源与环境科学

  Gradual Impacts of Climate Change on Rice Production in Fujian Province JIANG Min ,Jin Zhi Qing, , 2009, 25 (10):  220-227.  Abstract + ( 1751 )   PDF ( 708664KB )   ( 861 ) Abstract: Fujian Province is divided into three paddy rice planting areas with 19 sampling points been chosen. In the past 5 years from 2000 to 2004, yielding on odd years has been used to commission the CERES-Rice module parameter while yielding on even years has been used to check the module’s adaptability in the research area. The climate change of each sampling point in 2030 and 2050 has been generated referring to the output of GISS GCM Transient Run. CERES-Rice nodule has been run under all situations. The spice stimulations has been compared with that of the current climate and together with transpiration ration (β) and fluctuation of grain yielding (F), quantity analyzing is made to introduce the influence of gradual change of climate has on the production of rice in Fujian province; and based on this, certain possible solutions are forwarded. The research shows that in the process of climate change in the future, (1) the soil during the growth season of the rice in the research area would be less moist; (2) the production period of the early rice and single cropping rice would be shortened more or less while that of the late season rice would be prolonged in the climate situation of 2050; (3) the yielding of double cropping rice in southeast and northwest Fujian province would be lessened in both two climate situations in 2030 and 2050 with the production reduction rate increases when the temperature increases; and the yielding of single cropping rice in the mountainous areas of northwest Fujian province may enjoy a production enhancement; (4) as for the stability of yielding, currently those in the southeast of Fujian province is the worst and in the double cropping area in northwest Fujian is better; and the yielding will be less stable in the future climate change; (5) the total yield of rice in Fujian province will be decreased with the increase of temperature in both two future climate situations.

  Cluster Analysis on Phenological Type of 42 Kind of Plants in Minqin Desert Area Chang Zhaofeng,,, Han Fugui,,, Zhong Shengnian,, 2009, 25 (10):  228-234.  Abstract + ( 1238 )   PDF ( 991215KB )   ( 802 ) Research on phenology often needs to classification for studies the distribution and relations with environment. The system cluster consider only the integrated information of phenophase when to divide the type of phenophase, thus neglect often the part of the phenological information. In this article, phenophase type of the main phonological classified by cluster system, and the classification of phenophase merge by the main phonological. Thus overcome the defects of only focus on integrated information and ignore the differences between different phenological information when direct clustering by phenology, it was to ensure that pant is smallest classification unit at the same time. In this article made the cluster of phonological type by 4 of main phenophase with bourgeon period, leaf expansion period, flowering period and fruit ripenes period of observed data on phonology of 42 kind of plant 34 year in Minqin desert area, further the 23 of phenology classification merged by phonological type. The 23 of phenology classification basically obeys the ecotype characteristic of the plants, and the more scientific than the type of system cluster by plants sample. Moreover, the form of phonological type record by “ten day period + phonology” in the article. the form of phenology type record by “ten day period + phonology type — ten day period + phonology type — … ”, this is not only concrete, and direct-viewing, it is also advantageous for the application.

  Effects of cadmium on seed germination of different wheat varieties jy , , , , 2009, 25 (10):  235-240.  Abstract + ( 1451 )   PDF ( 500411KB )   ( 878 ) By using the techniques of laboratory bioassay, this paper studied the effects of 0.5 mmol/L of cadmium on the germination rate, germination index, vigor index, root length, shoot height and fresh weight of root and shoot to assess the cadmium-resistant ability of wheat by the synthetical effect with 30 wheat varieties mainly cultivated in the province of Guizhou. The results showed that after treated with 0.5 mmol?L-1 of cadmium, seed germination rate was less affected, but germination index, vigor index, root and shoot growth were restrained evidently. Cadmium had more serious impact on root than on shoot. Different wheat varieties had different germination responses to cadmium. Based on their responses to cadmium, the test 30 varieties were clustered into 3 types, ie, tolerant type, medium type, and sensitive type. Fengyou 5, Zaomai 16 and Fengyou 970 were tolerant to Cd and the sensitive varieties include Anmai 5, Chuanmai 10, Fengyou 2 etc.

  Study on the Main Vegetation Types and Species Abundance of Laoshan Mountain in Qingdao 2009, 25 (10):  241-245.  Abstract + ( 1668 )   PDF ( 551966KB )   ( 1089 ) The study on the community structure of the main vegetation types in Qingdao Laoshan mountain by setting typical plots for conservation and restoration of vegetation and increase of forest coverage .The study showed that there were various vegetation types,because the landform was complicated，and the climate was various, there were various kinds of plants, and introduction of the species.There were different vegetation types of different elevational gradients.The distribution of coniferous existed widely, Pinus densiflora was the constructive species.There were lot of deciduous broadleaved forest in the broadleaved forest,and there were Quercus acutissima、Quercus variabilis and so on in the deciduous broadleaved forest. The mesophyte or xeric mesophyte perennial herb was the constructive species in the Shrub-grassland;Taxa richness was different with the different elevation, showing a unimodalpattern along an elevational gradient;The species abundance was higher in Beijiushui,Weizhu’an,Taiqinggong,Liuqinghe,Yangkou and so on,and the species abundance was lower in Shuangshiwu,Lengcuixia,Guanlaocui and so on.

  Study on Physical and Chemic Characte of Forestry Soil Polluted by Heavy Metals in Antimony Mine of Len-Shuijiang City Tong Fanngping,, Xu Yanping, Long Yingzhong,, Yi Jianxin, Song Qing’an,, Yi Aiqin,, Shi Wenfeng, Li Gui, Zou Liwei, Dong Xiaohui 2009, 25 (10):  246-250.  Abstract + ( 1622 )   PDF ( 497281KB )   ( 1144 ) Abstract: The physical and chemic character of forestry soil polluted by heavy metals in antimony of Len Shuijiang city is studied and in order to afford scientific principle to ecological rehabilitate soil and select afforestation trees and restore vegetation rapidly. The result showed that there are significant difference in pH, soil organic matter, available nitrogen, available phosphorus, available potassium of antimony mineral sites. There are significant difference in soil bulk density, soil bulk density, soil organic matter, available phosphorus, available potassium of antimony mineral soil depth. The forestry soil of antimony mine is feebleness acid reaction. The soil bulk density, available potassium is maximum in Lianmeng plot. The soil organic matter, available nitrogen, available phosphorus is maximum in Qilipu plot. The soil bulk density increase and the soil organic matter and available phosphorus reduce with the depth increase but available nitrogen is polymorphic. The available potassium of topsoil maximum. The soil organic matter and available potassium is enrichment but available phosphorus is poor.

  热带农业科学

  Analysis on Rubber Tree Cold Injury in 2007/2008 winter in Hainan Kan Liyan, Xie Guishui, Tao Zhongliang, Yang Lifu, Cui Zhifu 2009, 25 (10):  251-257.  Abstract + ( 1274 )   PDF ( 1002316KB )   ( 689 ) The rubber tree cold injury in 2007/2008 winter in Hainan province had been investigated. By analyzing temperature climatic feature, and ecological types ,the effect of clones, age stages, landform and the location condition, it expands the knowledge on planting of cold resistance, and enriches the applying documentation on new rubber clone. Further, pertinence suggestion is put forward on cold protection.

  农业信息科学

  Study on the Limited Factor of Extension and Application to “Intelligent Decision System for Corn Production Management” Wu Yuhua, Zhu Yeping 2009, 25 (10):  258-263.  Abstract + ( 1179 )   PDF ( 509953KB )   ( 625 ) The thesis studied limited factor of extension and application to Intelligent Decision System for corn production management. The aim of this research is to determine the main factors affecting farmers’ adoption behavior of “Intelligent decision System” based on the data from the Nongan country and Yushu city in Jilin Province. In order to analysis the main limit factors of extension for this Intelligent Decision System, qualitative and quantitative analysis methods were adopted. The main factors which affect farmers’ adoption of the technology were determined by Logit Model.

  The research of new pattern of constructing informationize in farm of Xinjiang Production and Construction Group Wang Hongjiang, Lv Xin 2009, 25 (10):  264-267.  Abstract + ( 1335 )   PDF ( 468192KB )   ( 659 ) According to the mission of informationize construction of new pattern of farm in Xinjiang Production and Construction Group, we have analysed the status quo and problem of farm in Xinjiang Production and Construction Group, at the same time we have offered the developmental suggestion and constructive suggestion for constructing informationize of new pattern of farm and Xinjiang Production and Construction Group, while we have explored informationize construction of new pattern of farm in Xinjiang Production and Construction Group. We will utilize agricultural informationize to advance agricultural modernization when we actively construct digitale and networkable information in farm. Our suggestion will afford information technology and information system for development by leaps and bounds of Xinjiang Production and Construction Group.

  Design and Application of land management Information System in Wenzhou City based on MapX chen can , , 2009, 25 (10):  268-272.  Abstract + ( 1300 )   PDF ( 580162KB )   ( 736 ) The development goals and design principles of Wenzhou City land management information system were applied in this paper. The management information system modules, database development and the sub-function modules of overall Wenzhou City land were designed by WebGIS technical methodology. This system was developed by Mapx and MapInfo. After built this system, the integration of land data management had completed. This system included data editing, inquiring, automatically generating statistical analysis and data out. These results can provide some conveniences, scientific and effective methods for land managers to make decision.

  农村能源科学

  Study on Influencing Factors of Choosing Renewable Energy Biogas-----A Case Study on Rural Household Biogas in Jiangsu Province 2009, 25 (10):  273-276.  Abstract + ( 1373 )   PDF ( 538475KB )   ( 750 ) Based on the investigation for the consumption of the rural household biogas in Jiangsu , this paper concludes that per capita energy consumption of the family with biogas is lower than that without biogas, because biogas alternates biomass used by legging way and some commercial energy, and it also contributes to adjust the energy structure of raising the proportion of new energy and commodities. Furthermore, choosing biogas, for rural household, is influencd by many factors, of which the financial and technical support from government playes the biggest positive role.The raising of per capita amount of live pigs and the increase of the highest years of schooling of the staying-home population are positively related to the choice of biogas. However, if farmers live in major grain-producing area or use LPG, they will not choose biogas. And the per capita net income and proportion of the employed labourer in a family act negative role: with their increase, farmers declin the probability of choosing biogas.

  农业工程科学

  Risk Evaluation of Meteorological Disaster on Grain Yield in Fujian Province Chen Jiajin, Zhang Chungui, Wang Jiayi, Lin Jing, Li Lichun, Yang Kai 2009, 25 (10):  277-281.  Abstract + ( 1512 )   PDF ( 1435287KB )   ( 1002 ) Based on per unit grain yield derived from 66 counties during 1978-2004 in Fujian province, relative meteorological yield was analyzed with the help of statistic means, and the risk degree of meteorological disaster on grain yield was showed by the variety of relative meteorological yield in this area. We choose three comprehensive risk assessment indices: average rate of yield reduction and variation coefficient of reduction rate in the poor harvest year, Cumulate probability of relative meteorological yield less than -5%. Through the above indices, the risk of meteorological disaster of per unit grain yield in Fujian Province was evaluated.The result indicated that the comprehensive risk index of each county in Fujian Province between 0.08~0.73, the high risk region mainly distributed Pingtan county, the mostly counties of Quanzhou and Zhangzhou , the west counties of Longyan and Sanming, the northeast region of Ninde and some counties of Nanping, the per unit grain yield of this area is mostly affected by some meteorological disasters, such as typhoon, rainstorm, drought, cold injury and so on. The low risk region mainly distributed in littoral counties of middle region and the interior region of Fujian province; the effect of meteorological disaster in this area is smaller than the high risk region.

  Study on Field Experiment of Summer Corn for Low Border Irrigation Yang Xiaoyuan,,, Fan Xingke,,, Wu Pute,,, Ye Chengheng,, 2009, 25 (10):  282-286.  Abstract + ( 1471 )   PDF ( 586705KB )   ( 809 ) Abstract: Field experiment was carried out in Yangling area to study the effect of border irrigation on soil water and growth of summer maize under the condition of optimal soil water lower limit, Meanwhile, for purpose of rational utilization of water resources, exploring optimum methods and technical routes of low irrigation quota and forming limited irrigation system of crop high efficient use of water. The results shows that the variation of average soil water content was small but of soil water distribution in border check of different border dimensions was big. The optimization inflow rate of unit width，border wide and border length in field experiments are in the rang of 3～6l/m/s,2～3m and around 50m could ensure high irrigation quality with Application Efficiency(Ea>80%) and Distribution Uniformity (DU>80%). The variation of physiological characters of maize was small with different border dimensions but big with different irrigation quota in jointing-heading stage of mazie. Under the condition of border irrigation of different irrigation quota had a big effect on summer maize yield and water use efficiency.

  Analysis on frost events occurrence at Taiyuan from 1951 to 2008 , , 2009, 25 (10):  287-289.  Abstract + ( 1248 )   PDF ( 543760KB )   ( 670 ) Abstract: Based on the daily minimum temperature data at Taiyuan meteorological station from 1951 to 2008, the first and late frost events occurrences series were established respectively. Basic characterics were analyzed by using statistical method and the change trends of them were analyzed by using the linear method and the unusual frost were also analyzed by comparing them with criterion. The results were listed below: (1) The first frost event occurred on Oct. 14th, the late frost event occurred on Apr. 15th and the frostless season was 182d on average. (2)Comparing with the average date, the first frost events occurred early in 1950s, 1960s and 1990s, normal in 1970s,late in 1980s and early 21st century; the late frost events ended late and frostless seasons were short in 1950s-1970s.After 1980s, they were early and long (3) The first frost events occurred towards a late trend obviously(2.2d/10a), the last frost was a ahead trend(-1.8 d/10a) and the frostless season was longer and longer. (4)The frequency of unusual first frost events took up 17.2% (including 1.7% for earliest and 15.5% for earlier respectively) and that of unusual late frost events took up 12.1%(including 5.2% for latest and 6.9% for later respectively).

  水产渔业科学

  Effects of Starvation and Refeeding on Digestive Enzyme Activities of Brachionus plicatilis Guo Enmian, Wang Xin, Chen Xiaohua, Song Jialei, Zhang Yanping 2009, 25 (10):  290-295.  Abstract + ( 1448 )   PDF ( 1708456KB )   ( 726 ) Several digestive enzyme activities of the rotifer Brachionus plicatilis during starvation and refeeding were studied under 25℃. The results showed that activities of amylase and cellulose maintained high lever during the whole process. During starvation, activities of amylase and cellulose decreased dramatically in the first two days, and then maintained stable, while activities of pepsin and tryptase decreased slowly. When egg ratio was less than 0.09, all four digestive enzyme activities were lower than the compared. After one-day refeeding, egg ratio increased from 0 to 0.21, activities of tryptase and cellulose increased to normal level, and activities of pepsin and amylase were higher than the compared. Stomach and gastric glands restored normal after one-day refeeding in vivo observation. It was found these four digestive enzyme activities were not related with the egg ratio when rotifer was fed.

  三农问题研究

  Research on the Development of Agriculture in China from 1978 to 2008Ⅰ．Achievements 2009, 25 (10):  296-299.  Abstract + ( 1438 )   PDF ( 468657KB )   ( 660 ) Since 1978 to 2008, the policy of reform and opening-up in China has carried out 30 years. In this stormy 30 years, China agriculture has undergone enormous changes, has scored great achievements. The great achievements of China agricultural development in increasing of production, improving of agricultural product quality, optimizing of agricultural structure, increasing economic value, increasing income of farmers, eradicating poverty, transferring rural labors, increasing agricultural models, raising agricultural function, making the progress of agricultural scientific and technologies, upgrading the quality of the peasants, building of ecurity system, developing of public utilities, and enhancing the international competitiveness of agriculture have been reviewed and concluded in the paper, which has the reference value for the marching of China agriculture in new stage of new century.

  Research on Agricultural Sci-tech Team Promoting Building of a New Socialist Countryside Mao Jian, Wang Hui, Xu Dasheng, Zhou Dawei, Yu Ning, Fu Anjing, Shi Wei 2009, 25 (10):  303-306.  Abstract + ( 1309 )   PDF ( 450377KB )   ( 647 ) Based on Team Theories, this paper introduces the exploration & practice on sci-tech team construction and team innovation of our college in the course of building a new socialist countryside. During team building, our college has made remarkable achievements in scientific research, technology popularization, serving local economy, promoting the building of a new socialist countryside and so on. This research would provide helpful references for agricultural colleges and scientific research institutes to serve agriculture, rural areas, farmers and the building of a new socialist countryside.