Abstract: On the basis of guanidine thiocyanate method, an economical method of extracting and puring total RNA from Yunjie stigmas was established.The results showed that the total RNA were in good integrity and high purity, which demonstrated that RNA samples were free of contamination of protein,DNA,polysaccharide and polyphenol ,and were applicable to cDNA synthesis,DDRT-PCR,northern blotting and so on.This method was simple,economic, and of stable performance, good repeatability as well as suitable for extracting total RNA from many materials.