Abstract: Abstract ：【OBJECTIVE】Salicylic acid (SA) was found to improve biotic and abiotic tolerance of plants. In present paper cucumber “Xinyou30” was employed to study the effect of SA on plant gene expression and its mechanism. 【METHOD】Under chilling stress at 7℃ cucumber seedlings were sprayed by distilled H2O and SA (3mmol/L, pH6.0), respectively. After 48 hours total RNA was extracted from cucumber leaves and reversely transcripted into first strand cDNA. mRNA differential display was applied to analyzed SA-induced cDNA in cucumber leaves. 【RESULTS】Twelve SA-induced cDNA fragments were isolated and retracted. Two （SICCL_1 and SICCL_2）of them were affirmed by reverse dot blot to be significantly induced by SA. Nucleotide sequence analysis showed that SICCL_1 had 88% identity to AM734282 related with Cucumber Mosaic Virus (CMV ) in Cucumis melo subsp. melo; SICCL_2 had86% identity to THL24h-106 related with NaHCO3 stress in Tamarix hispida. 【CONCLUSION】It is postulated that SA may improve tolerance of plants via inducing overlapping genes against biotic and abiotic stresses.