Abstract: In order to study the heterosis of tobacco hybrid F1 generation, 8 materials of novel tobacco Cultivars, which include Zisu tobacco, Mantuoluo tobacco, Luole tobacco, MSG-28 tobacco, G-28 tobacco, M.Zisu tobacco, M.Mantuoluo tobacco, M.Luole tobacco, are recorded by observing their duration period, main agronomic characteristics, and their production quality and quantity situation. Through analyzing those data, it shows: those F1 generations do have the strong heterosis in the seedling stage. However, during other stages of growing in fields, there is no outstanding superiority. The reason why the superiority is not obvious during the later period may caused by the external environment.