Abstract: Abstract: Immature embryos of a common wheat variety Linyou145 was used as explant to induce regeneration and transformation,respectively. The immature embryo were inoculated on the MS medium with 2 mg·L-1 2,4-dichlorophenoxyacetic acid to initiate calli. Then,they were inoculated on the MS medium with 1mg·L-1 ZT and 1mg·L-1 IAA, cultured for 2-3weeks for plant differentiation.Finally,the calli of differentiation were transferred into the MS medium with 0.5mg·L-1 NAA for plant regeneration.Following the above protocol,987calli,123resistant callus and 34 plantlets were obtained from 1229 embryosintotal, Furthermore, plasmid DNA of pCAMBI1301 containing GUS gene as the donor of alien gene was transformed into the immature embryo calli by Agrobacterium tumefaciens.By means of PCR amplification, hygomycin resistant plantlets were obtained. The result from X-gluc staining showed that GUS gene gave transient expression successfully in the transformed calli.Blue spots were observed in resistant calli.