Abstract: The characteristic of chemical components of flue-tobacco leaf were studies by detecting total sugar, reducing sugar, nicotine, total nitrogen, potassium and chlorine of 90 flue-tobacco samples with 3 grades(B2F,C3F,X2F) from the typical tobacco-growing town of Zhangjiajie City. The results showed that total sugar, reducing sugar, total nitrogen, chlorine were at right levels, and nicotine was slightly high, and potassium was slightly low in top leaf. The concentration difference of chemical components in tobacco samples between grown years was significant, but that between tobacco-growing counties and between tobacco-growing town was not statistical significant. The main chemical components of flue-cured tobacco leaves in 6 typical tobacco-growing towns of Zhangjiajie City were divided into 2 types(high sugar and potassium, low sugar and potassium) by the cluster analysis.