Abstract: Cultivation in Greenhouse has become one important cropping system in Yunnan province. The accumulation of salt caused by different fertilization may affect the soil ecosystem in Greenhouse. This paper investigated the salt contents of soil surface horizon in Greenhouse soil from six major areas in Yunnan Province. The results are: In the top layer of Yunnan greenhouse soil, the soil total N, P and K contents were 0.99-4.15 g•kg-1, 0.53-5.64 g•kg-1, 1.15-56.49 g•kg-1 respectively. The available N, P, K contents were 69.0-368.9 mg•kg-1, 6.95-214.9 mg•kg-1, 72.8-2654.4 mg•kg-1 respectively. With the increasing of the greenhouse ages, total N, total P, available N, available P and available K of soil appeared increased. Soil nutrient accumulated in the top layer, the contents of soil nutrient decreased gradually with the soil layer deepening．