Abstract: The aim of this paper was to study the composition and polymorphism of Glu-1 Subunits of wheat varieties that selected from liberation to now in Shandong province and select breeding material to improve wheat quality. The Composition and Polymorphism of Glu-1 Subunits were analyzed by SDS-PAGE. The results showed that “null” and “7+9” were the predominant allele variation patterns on Glu-A1 loci. Five allele variation patterns, “7+8”, “7+9”, “14+15”, “17+18” and “6+8” were detected on Glu-B1 loci, but the predominant patterns were “7+8” and “7+9”. Six allele variation patterns, “2+12”, “2+10”, “5+10”, “4+10”, “4+12” and “2+11” were detected on Glu-D1 loci, but the predominant pattern was “2+12”. In those three loci, Glu-B1 loci had the highest polymorphic frequency, Glu-D1 loci was second, and the last was Glu-A1. The polymorphism index of Glu-1 decreased gradually from 1950’ and reached to the lowest point in 1960’. After that the polymorphism index of Glu-1 increased slowly and got the highest point in 1990’. But the index showed decrease from 1990’ to now. The frequencies of the high quality subunits were low in wheat variety and its parent in Shandong province. This could be a way to improve wheat quality of Shandong province.