This experiment investigated the cold-tolerance in two japonica drooping leaf mutants(MR304、MR312) and its crossing progenies (F1 and F2) at seedling stage under the high elevation cold area in Yunnan, analyzed the pollen fertility between the mutants and their crosses at heading stage, and measured the correlated economical and biological traits at maturity stage. The results showed that: mutants and parents were resistance (R); the Nan29/MR312, Dianjingyou5/MR304, Ansanbyeo/MR304 were medium resistance (MR), the Dianjingyou5/MR312 was sensitivity (S), others were resistance (R) in F1 The pollen fertility of Yinguang at the heading stage were highest, which was up to 88.9%; the Yinguang/MR304 surpassed the contrast (92.5%) were the highest in F1 of nine combinations, to 94.5%, and Ansanbyeo/MR304 were the lowest. The seed-set rate of Yinguang surpassed the contrast (70.9%), to 82.4%, and seed-set rate of MR304 and MR312 were lower, 26.2%and 5.0%, respectively, and the seed-set rate of two combinations (Yinguang/MR304 and Yinguang/MR312) in F1 surpassed the contrast. The study revealed that we could utilize the F1 heterosis derived from the cross of drooping leaf mutants and conventional varieties to enhance the superiority of cold tolerance in the high elevation cold area, and the pollen fertility at the heading stage was the important index to evaluate the cold tolerance in crossing progenies F1.