Abstract: SSR marker-based gene mapping of Rf3 for fertility-restoration of rice wild-cytoplasmic male sterility was conducted using an F2 population derived from the cross Zhenshan 97A / Minghui 63. The mapping was performed using the recessive class consisting of 119 extreme sterile individuals. Parental polymorphism survey was conducted using 37 SSRs located in the probable region for Rf3 on the short arm of rice chromosome 1. Of the 16 polymorphic SSRs detected, nine were selected to test the extreme sterile individuals. RM10353, RM1195 and RM3746 which were located consecutively in the segmental map each showed eight single crossover with Rf3, and this number was the lowest among the numbers of recombinant observed between the nine SSRs and Rf3. This result indicates that Rf3 was located in the genomic region covered by interval RM10353-RM1195-RM3746 and its flanking markers. Thus, the Rf3 locus was mapped between RM10338 and RM10376 that have a physical distance of 679.9 kb.