Abstract: The regions of high sand soil along the Yangtze River is main productive area for weak-gluten wheat in Jiangsu province and in china. Ningmai 9 and Ningmai 13 were weak-gluten wheat varieties, which exhibited difference in grain yield, panicles, filled grains per panicle and 1000-grain weight under different fertility levers, and also difference in those characters on Ningmai 9 and Ningmai 13 under different fertility levers. The grain yield of Ningmai 13 was significantly higher than that of Ningmai 9 under 16 Kg pure N per 667m2, and its significantly less under 8 Kg pure N per 667m2 and no nitrogen Management. there was no significantly difference of grain yield between Ningmai 9 and Ningmai 13 under 20 Kg pure N and 12 Kg pure N per 667m2. Correlation analysis showed that there was significantly difference bewteen grain protein content, dough stability time and nitrogen fertilizer rate on Ningmai 9, and no significantly difference bewteen wet gluten content and nitrogen fertilizer rate. There was significantly correlation bewteen grain protein content, wet gluten content, dough stability time and nitrogen fertilizer rate on Ningmai 13. The results revealed the quality of wet gluten to Ningmai 9 was more stability than that of Ningmai 13.