Abstract: We selected activated carbon , slag, ceramsite and volcanic as the substrates of vertical flow wetlands and studied the effect of different substrates on livestock and poultry breeding waste as well as the relation between the substrates purification effect on polluted water and time. The results indicate that all of the three substrates had good purification effect on total phosphate (TP), the maximum degradation was 99.6﹪,79.8﹪ and 92.3﹪ respectively, meanwhile three of all had good degradation effect on turbidity and they were 98.5﹪,90.6﹪ and 82.8﹪ respectively. Among substrates the mixture of activated carbon and slag needed more COD(biological oxygen demand) and had better degradation effect of TN (total nitrogen) than ceramsite and volcanic, they achieved 93.2﹪ and 68﹪ while volcanic and ceramsits can get to 58.6﹪ and 53.6﹪ for COD’s degradation respectively, but both maximum degradation of TN only got to 38.2﹪ and 14.4﹪,also their degradation of NO- 3 were 74.1﹪and 61.3﹪respectively. For the time span from 0to 5 days, criteria of polluted water reduced quickly then the purification effect of polluted water trended to be stable.