Abstract: To breed new hybrid rice with intermediate level amylose content (AC), a new rice variety with intermediate AC, HC086, was used as the donor parent of Wx gene, crossed with 4 restorers (R898, R476, R838, R6547) to raise their AC, combining the marker-assisted selection (MAS) of PCR-AccI testing technology. Analysis of genotypes and AC of improved lines revealed that the AC of new lines with new Wx gene were elevated from10.6-14.4% to 18.0-21.4% , and the F1 hybrids derived from new lines crossing to Guangzhan63S and Y58S reached to the intermediate AC level with no obvious change in main traits.