Abstract: Research on the difference of the fertilizer-N agronomic use efficiency (AEN), grain yield and the rice quality by using six varieties under five levers of N-fertilized. Results showed that the grain yield was improving with the amount of the N-fertilized increasing. Under the different levers of N-fertilized, the AEN were difference. The AEN was decreasing with the amount of the N-fertilized increasing. But, different varieties have a different result under the different levers of N-fertilized. The AEN of the Xieyou 57 was the highest under the lever of 75 Kg N-fertilized. At the difference yield objects, the object could be achieved by using the difference varieties. But, the risk of Xieyou 9019 was the lest. Because its average AEN was the highest. Duochandao and the Shanyou63 were the varieties, which could endure the lower lever of N-fertilized. At the amount of 150Kg N-fertilized, the rice quality could be improved.