Abstract: Using the improved method of CTAB，high purity DNA was obtained from banana leaves．Based on the common RAPD reaction program and adjusting experiments，the optimal amplification program was described as follows：94℃ for 5min；45 cycles at 94℃ for 1 min，38℃ for 1 min and 72℃ for 2 min；72℃ for 2min at last. PCR system（25μL total volumes）contains：20ng of genomic DNA，200200μmol/L of dNTP，2.5μL of 10×PCR Buffer，0.20μmol/L of primer，0.75U Taq DNA polymerase，17.85μL of ddH2O． Key words：banana；Template DNA；RAPD