Abstract: Based on their characteristics of haploid and high embryogenetic capacity, plant isolated microspores and microspore-derived tissues are the good acceptors in plant genetic engineering, by which the homozygous transgenic plants could be obtained more quickly. The applications of plant isolated microspores, microspore-derived embryos and microspore-derived calli are reviewed and their existing problems are discussed in constructing genetic transformation system, including the detailed transformational technology of Agrobacterium mediation , particle bombardment, laser microbeam puncture, microinjection, electroporation, polyethylene glycol(PEG)-mediated.