Abstract: Abstract: The sixth generation of hops used in vitro were used to study the differentconcentrations of auxin, medium nutrients and carbon sources of glucose concentration on in vitro rooting of the effects of transplanting. The results show that the IBA concentration of 0.05mg / L is best to take root; not rooted in the culture medium B5(substantial) + MS (micro-, organic matter), the Organization of substantial inhibition leggy; when the medium glucose concentration of 15%, seedling the rooting rate and the highest survival.