Abstract:

Through synthesizing a part of specific primers, the PCR was established by means of the template of DNA from SS2 Fujian strain, We developed the PCR method to detect Streptococcus suis serotype 2(SS2) from pig, and the CPS2J partial gene was sequenced and blasted. The results were: The 675bp fragment from SS2PFJ06 and standard positive SS2 was amplified by the PCR, as well as expecting fragment. However, 8 strains not SS2 were not amplified to the 675bp fragment. The PCR detected less 100cfu of bacterium quantity or 50pg of genic DNA through the sensitive test. The blast of the sequence of PCR product achieved to 98%-100% homology with that of other SS2 strains, amino acid sequence achieved to 91.6%~93.3%. The results reveals the PCR developed were sensitive and specific and applied to the SS2 diagnosis and epidemiology investigation. Sequence analysis indicated homology between SS2 Fujian strain and 8 SS2 at home and abroad was high and genetic relationship was close.