Abstract: The zinc finger proteins belong to the largest family of regulatory transcription factors, which play an important role in growth and development in animal and plant systems. In this study, a novel zinc finger gene OsZNP was isolated from rice cDNA library by RT-PCR approach. Sequence analysis indicated that the gene encodes a 28 kDa protein with 253 amino acids and contains one typical C3HC4 zinc finger domains. The entire coding region of OsZNP gene was amplified by PCR. Then it was inserted into the prokaryotic fusion expression vector pGEX-KG to construct recombinant expression vector pGEX-KG-OsZNP, which was transformed into E. coil BL21. After the transformed bacteria were induced with IPTG, the expressed protein was analyzed by SDS-PAGE. The results showed that the target protein was expressed at a high level in E.coli BL21．