Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2009, Vol. 25 ›› Issue (13): 150-153.

• 园艺园林科学 • Previous Articles     Next Articles

Preliminary Study on Adventitious Bud Regeneration from in Vitro Non-bud Stem of Ziziphus jujuba Mill.‘Fupingdazao’

Zhou Xiaoli, Peng Jianying, Duan Hongxi   

  • Received:2009-04-02 Revised:2009-05-07 Online:2009-07-05 Published:2009-07-05

Abstract: The factors affecting adventitious bud regeneration from in vitro non-bud stem of Ziziphus jujuba Mill.‘Fupingdazao’ in MS media were studied, which included sterilization time, dark incubation time and plant growth regulator. The results showed that 8-10 minutes of sterilization time for young non-bud stem was suitable; 21 days of dark culture was optimal ;The best combination of plant growth regulators was confirmed on MS+6-BA 1.0mg.L-1+ IBA 0.1mg.L-1,on which regeneration rate and Adventitious buds per stem were 85.42% and 3.23 respectively, they were significantly higher than other treatment, and the adventitious buds could become developmental branch.