Abstract:

Abstract: The population was constructed using 733 wheat germplasm originating from 6 countries including China, Australia, Mexico, Russia, Netherlands and Chile. We evaluated the effect of 19 different sampling methods on traits means, variance coefficients and diversity index using country as subgroup. The results indicated that there were no significant effect on trait means and variance coefficient when the sample size below 60 using equilibrium sampling method, while there were significant effect on diversity index. There were no significant effect on means, variance coefficients and diversity index when the sampling size bigger than 120. Increasing sample size can reduce the effect of sampling method on variance coefficients and diversity index when the sample size above 150. Decreasing the ratio of subgroup difference can reduce the effect of disequilibrium sampling method on variance coefficients and diversity index when the sample size between 90 and 150. The effect was small to the means, variance coefficients and diversity index when the number of subgroup above 20. The accuracy can be improved by increasing the number of subgroup.