Abstract:

To establish appropriate longan SRAP-PCR reaction system, factors that affected the amplified result of SRAP such as amounts of DNA and Taq DNA polymerase, concentrations of Mg2+, dNTP and primer were studied by staged optimization method. Reaction system suitable for SRAP analysis was established. The reaction mixture (25μl) contained, 1 ×PCR Buffer, 2.0mmol/L Mg2+, 0.5 mmol/L dNTPs, 0.3μmol/L primer, 10 ng DNA template, and 1.5 U Taq DNA polymerase. The results showed the reaction system could meet SRAP amplification requirement of longan genome, and SRAP marker for longan genetic study was feasible.