Abstract:

Abstract: The acetyl-CoA carboxylase (ACCase) is the rate-limiting enzyme of fatty acids synthesis.The accD Gene and transit peptide of CAC3 Gene was amplified from Gossypuum hirsutum Genome and Arabidopsis thaliana Geneome. Plant Expression Vector of Fusion Transit Peptide of CAC3 Gene and accD Gene was constructed.The Vector of pBI-CAC3tp-accD were transferred into Agrobacterium tumefaciens GV3101. Using infiltration and leaf discs method, the gene were transferred into Arabidopsis thaliana and tobacco cell. The seeds of Arabidopsis thaliana were selected on solid medium containing Kanamycin. Transferred leafs of tobacco were selected on solid medium containing Kanamycin. Transgenic Arabidopsis thaliana (T1) and tobacco plants were found containing purpose gene by PCR. After RT-PCR, it shows that the CAC3tp-accD gene could transcript normally.