Abstract:

Abstract: THRSP gene is a important gene which involved in the regulation of animal lipogenesis.In this research,RCR-SSCP and DNA sequenscing were used to analysis the Polymorphisms of THRSP gene part exon1 in 644 cattle which comes from Qinchuan cattle (QC),Xianan cattle (XC),Nanyang cattle (NC),Jiaxian Rec cattle (JRC),Luxi cattle (LC).It was detected two mutations that is 40bpC/T and 78bpA/G and four genotype were fouded which is AA,AB,BB,AC. The allele frequencies is likely in five cattle breeds.In He,QC﹥XC﹥LC﹥NC﹥JRC;in PIC,QC belonged to high polymorphisms (PIC﹥0.5）, XC, LC, NC, JRC belonged to moderate polymorphisms（0.25﹤PIC﹤0.5）. The 40bp C/T mutation leads to a missense mutation from Val(GTG)→Ala(GCG), The 78bp A/Gmutation leads to a missense mutation from Val(GTC) →Ile(ATC).