Abstract: Abstract：The seedlings of wheat were exposed to He-Ne laser irradiation after enhanced UV-B radiated,in order to determine some physiological traits in the damage induced by UV-B radiation. The seeds were cultivated in Petridishes in a constant temperature incubator(25℃)until they were germinated.The germinated seeds were exposed to 10.08 kJ?m-2 UV-B radiation, one by one, and then they were irradiated by He-Ne laser with 10mW?mm-2 Power density.Changes about the polyamine contents (PAs) and the activity of the polyamine oxidase(PAO) and the content of MDA were measured to test the repair role of laser irradiation. The results showed that the damage of wheat seedlings induced by enhanced UV-B radiation in physiology can be repaired partly by He-Ne laser irradiation with decreasing the content of PAs and raising the activity of PAO. This is the first investigation reporting the use of laser irradiation treatment to repair the wheat damage induced by UV-B radiation in peroxidase and polyamine oxidaset.