Abstract: The physiological and biochemical characters of different mulberry varieties were studied in this experiment, of which the mulberry varieties Premium 2, Guangdong Mulberry 11 and Guisang 62 which are major mulberry varieties cultivated in Yizhou were used as materials. The results showed that, in different testing time the chlorophyll content of leaves of Premium 2 was higher than that of Guangdong Mulberry 11 and Guisang 62. The soluble sugar content and water-soluble protein content of leaves of Guangdong Mulberry 11 than were higher than that of Premium 2 and Guisang 62. The water content and dry matter weight of three mulberry varieties reached the normal level in different testing time. The MDA content of leaves of Premium 2 was the lowest, while the proline content of it was the highest.