Abstract: Abstract: Artificial antigen of acrylamide was synthetized by couple method, and anti- acrylamide polyclonal antibody were obtained by immunizing method. The Glutaraldehyde method was used to conjugate Acrylamide to Bovine Serum albumin(BSA), and artificial antigen, acrylamide and BSA were respectively taken on UV spectrum scan. Rabbits had been immunized with artificial antigen, and the titer antiserum from those rabbits were testified by indirect enzyme-linked immunosorbent assay; The result showed that artificial antigen was synthesized successfully between Acrylamide and BSA, and titer of anti-acrylamide serum was above 8100; Anti-Acrylamide polyclonal antibody was prepared by immunizing method of artificial antigen.