Abstract: The in vitro digestibility of lotus-seed starch and lotus-seed starch edible film were studied by In-Vitro digestion model simulating human intestinal system. Glycemic index (GI) was conjectured according to the percentage of starch hydrolysis. The results show that the rates of digestion of lotus-seed starch and lotus-seed starch edible film with heat treatment were greater than for the sample without heat treatment. The hydrolysis rates of lotus-seed starch edible films were higher than for lotus-seed starches. Glycemic Index of lotus-seed starch edible film was greater than 90, which belongs to high GI foods.