Abstract: In this paper,Sophora flavescens ait,Coptis chinosis franeh,Asarum,Radix isatid, Aloe carried out on toxicity measurement for the Ceratocystis fimbriata,the results showed that five kinds of plant have inhibition to it,and it’s bacteriostasis rate was 93.33%、97.78%、71.11%、64.44%、66.67% in growth adaptation period,the best bacteriostatic effect plant was Sophora flavescens ait; In the strong period of growth,the bacteriostasis rate was 90.12%、86.63%、79.65%、68.03%、64.54%, the best bacteriostatic effect plant was Coptis chinosis franeh. This study found natural plant extract contain some active substances which can inhibition the pomegranate wilt disease fungus, this do provide a new way and method for the biological control of pomegranate wilt disease.