Abstract: The trial use the stems of Chenopodium L. for the tissue culture. With MS as the basic medium, after disinfection method, the proliferation of media, rooting medium screening, the test results shows that 75% alcohol 5 s +0.1% mercuric chloride 8 min to conduct a thorough disinfection, and ensure a higher survival rate ;MS+6-BA 1.5mg?L-1+ NAA 0.2mg?L-1+ agar 0.8% + sucrose 2% + activated charcoal 0.2% for primary culture, MS +6-BA 2.0mg?L-1+ NAA 0.2mg?L-1+ agar 0.8% + sucrose 2% + activated charcoal 0.2% for subculture, 1 / 2MS+IBA 1.0mg?L-1+ NAA 0.1mg?L-1+ agar 0.8% + sucrose 2% + activated charcoal 0.2% for the growing of roots, rooting rate of 80%.When the plantlets with 3 ~5 leaves, the height of 2~3 cm and 3 to 4 roots were transferred onto the mixture of Lennilite and Rotten breed soil(1∶2), and the survival rate was up to 90%.