Abstract: The effects of five organic fertilizers (OF) on flue-cured tobacco's metabolisms at early stage of flourishing grows were studied by pot experiments in the yellow mud fields. Results showed that the NRase activity of tobacco leafs all increased with the increasing of organic nitrogen (ON) in OF. The photosynthetic pigment content increased with the increasing of ON under chicken manure.The soluble protein and free amino content increased with the increasing of ON under chicken and straw combined application, but cake fertilizer, cattle manure, pig manure got opposite results. manure, cake fertilizer and straw, but cattle manure and pig manure got opposite results. And the total soluble sugar content enhanced with the increasing of ON under chicken manure, cake fertilizer and cattle manure, on the contrary pig manure and straw got opposite results. Because of various ON ratios in different OF, so the combined application rate of chicken manure, cake fertilizer, cattle manure, pig manure and Straw should be different.