Abstract:

Abstract: According to two conservative amino acid regions of Rar1 gene from different crops, degenerate primers were designed to amplify Rar1 fragment of chewing cane in this study. By the sequence alignment, the Rar1 fragment of chewing cane cloned from chewing cane cDNA had high similarity with the Rar1’s CCCH and CHORD-Ⅱregions of other crops. Rar1 fragment was constructed plant antisense expression vector pCAM-RAR1. The antisense gene was integrated into the genome of chewing cane callus via Agrobacterium tumefaciens EHA105 mediation. It provided a reliable base for the function s tudy on chewing cane Rar1 gene.