Abstract: Abstract: Based on the leaf-blades of Ramie being for the outside plants body, this paper researches on the duration of disinfection, culture medium and the growth of adjustment materials which affect the leaves of Ramie’s callus induction, the occurrence of adventitious buds and rooting. The result shows that, firstly, using 75% of alcohol to have disinfection in 10 seconds; then, using 0.1% mercuric chloride to have sterilization in 8 minutes,dripping into 1-2 drop of 1% HCL at the same time, it is the optium way to cause effective result. The best leaves of Ramie’s callus induction basic culture medium is 1/2MS. The best leaves induction organization culture medium combination is that: 1/2MS+0.05mg/L TDZ+0.01mg/L IAA+0.03mg/L 2,4-D; The optium injury differentiation medium is that: 1/2MS+0.5mg/L TDZ+0.02mg/L 2,4-D+0.03mg/L IAA; The best rooting medium is that: 1/2MS+0.02mg/L TDZ+O.O5mg/L NAA+0.02mg/L IAA. Thus, it has formed the more perfect tissue culture regeneration system, regeneration rate has reached 26% the above.