Abstract:

【Abstract】　Salmonella is an important foodborne pathogenic bacteria in animal food, establish a rapid detection methods plays an important role in animal food quality control. Three protocols were used to extract total DNA from one food sample from animal，and DNA products from the three protocols were all amplified by the primer of 16S rRNA gene and dT fermentation with Real Time PCR. Results show that this method is simple, rapid and high sensitivity, the detection sensitivity in animal food can reach up to 10 CFU/g, suggeste such method may be used fast testing the salmonella pollution in animal food.