Abstract:

A pair of primers was designed according to the upstream sequence of the flagellin gene of Bordetella bronchiseptica, which specifically amplified a fragment of 237 bp in length of the target gene. Specificity assays revealed that the assay didn’t cross react with Escherichia coli, Actinobacillus pleuropneumoniae, Pasteurella multocida, Streptococcus suis and Haemophilus parasuis. Sensitivity assays showed that the expected sequences were obtained from B. bronchiseptica individual colony diluted to 105 times. The results showed that this PCR technique was specific and sensitive, could apply to Bb diagnoses and epidemiology investigation.