Abstract: Plasmid DNA of pBILGC containing Chitinase and β-1, 3 - glucanase gene and fresh pollen of Papaver nudicaule were mixed in 11％ sucrose solution, then the pollen suspension were pollinated on stigmas of the Papaver nudicaule by artificial pollination. Transformed plants were primarily confirmed by PCR amplification and kanamycin selection，trangenetic T1 plant were obtained．The experimental results indicated that exogenous gene could be transformed by the Pollen tube pathway Transformation, and the new breeding method of Papaver nudicaule was provided.