Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2010, Vol. 26 ›› Issue (1): 32-35.

Special Issue: 生物技术

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The effect of organic compounds on the tissue culture of Cattleya

  

  • Received:2009-08-07 Revised:2009-09-11 Online:2010-01-05 Published:2010-01-05

Abstract:

The Cattleya labiata MC1 PLB were inoculated on the reformed VW + 5.0 mg/L 6-BA + 0.5 mg/L NAA + 20 g/L srose + 16 g/L agar medium adding six different organic compounds (Coconut milk、coconut juice、banana mud、Potato、Casein hydrollysate、Apple juice) with different concentrations (10%,15%,20%). The results showed that, the promotion effect of these six organic compounds from large to small successively is, Coconut milk、Potato、Coconut juice、Banana mud、Apple juice、Casein hydrollysate. The 15% Coconut milk was the best to the propagation of Cattleya PLB, with the highest propagation rate and the lowest differentiation rate. The ages of the PLB also affected the propagation of the PLB. The younger the PLB were, the higher the propagation rate was, and the lower the differentiation rate was.