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Chinese Agricultural Science Bulletin ›› 2010, Vol. 26 ›› Issue (1): 1-4.

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Initial Study on anti-bacterial activity of Mentha spicata Linn extraction in vitro

  

  • Received:2009-08-07 Revised:2009-08-30 Online:2010-01-05 Published:2010-01-05

Abstract:

Abstract:[Objective]To investigate the antibacterial function of Mentha spicata Linn extracts on Escherichia coli、Salmonella、Enterococcus、Staphylococci in vitro.[Method]In the study, the agar Cup-plate method was used to mensurate the size of antibacterial zone of Mentha spicata L. extractions, and the tube doubleness dilution method was used to determine their Minimum inhibitory concentrations (MIC)for 4 animal bacteria strains. [Results]The aqueous extract, the alcohol extract and the volatile oil of Mentha spicata L. has inhibitory effect to the selected strains. All of them have the best inhibitory effect on Staphylococci. The MIC of aqueous extractⅠ(sinking with alcohol )is 0.0313g/ml. The MIC of alcohol extraction Ⅲ is 0.0625g/ml . The MIC of volatile oil Ⅴ is 2.5μl/ /ml. The MIC of volatile oil Ⅵ is 2.5μl/ /ml.[Conclusion] Mentha spicata L. shows anti-bacterial effects on the experimental strains in vitro. Each kind of mentha spicata extracts has distinctive inhibitory effect to different bacteria.