Abstract:

The mature embryo of Pinus thunbergii×P. densiflora was used as explants to induce adventitious buds and form plantlets. The Paper studied the use of basal mediums, hormones, different display methods, different explants derived time, the concentration of different inorganic salts medium and subculture the proliferation of adventitious buds of Pinus thunbergii×P. densiflora proliferation and growth. The results showed that the best medium for adventitious bud inducement is WPM, the adventitious buds proliferation rate was above 3.01;.WPM adding with 2~3mg.L-16-BA and 0.01~0.05mg.L-1NAA for a nearly 76% induction rate. The mean proliferation rate of adventitious bud reaches 3.55. and Vertical display of adventitious buds better differentiation.the right stage of seedling was 21~28d. 3/4WPM good effects induced by inorganic salt concentration. Following the value-added and the best medium were 3/4WPM with 0.5 mg.L-16-BA and 0.02 mg.L-1NAA.