Abstract:

To develop a sicentific method for Glehnia litteralis genetic diversity analysis. Glehnia litteralis DNA was used to optimize SRAP system’s main parameters. A stability and reliable SRAP-PCR system was established. In the 20μL system, DNA 80 ng, Mg2+ 2.5 mmol/L, dNTP 0.25mmol/L, Taq 1U, Upstream and Downstream primers both for 0.25μmol/L. The reaction system was suitable to study Glehnia litteralis genetic diversity analysis and gene mapping.