Abstract:

[Abstract] [Objective] The research aimed to screen out optimum RAPD reaction system on genomic DNA of the leaves of Tripterygium Wilfordii Hook..[Method] The improved CTAB DNA extraction method，to extracted the genomic DNA of the leaves of tripterygium wilfordii Hook. The single factor experiment was adopted to select the required 5 annealing temperature，dNTPs concentration，template DNA concentration，Mg2+ concentration，Taq enzyme concentration and primer concentration.[Result] The optimum reaction system for RAPD amplification of the leaves of Tripterygium Wilfordii Hook. was listed as follows：3790 ng/L DNA，1.1μmol/L primer，1.25mmol/L dNTPs and 5.625 U/L Taq enzyme, the optimum temperature is 56.8℃.