Abstract:

This study intended to establish a culture system that would induce explant sterilization and initial medium. The stem of hybrid pine was 2 years old, which seeds were made of Pinus elliottii across Pinus caribaea of Australia. The results showed that the best way of explants sterilization was as follows: stem was washed in water for 5 minutes, in sterile water with tween-20 for 1 minute, in 70% ethanol for 10 seconds, in 0.1% HgCl2 for 6.5 minutes, and washed in sterile water for 5 times. Selection of the initial culture medium was with MS, 1/2 MS, GD, DCR , WPM and N6, which were common culture mediums on pine. 6-BA and NAA were also added to the selected medium. Inductivity, proliferation, elongation and root rate of bud were as the evaluating indicator. The better culture medium of hybrid pine was GD+BA(0.1－1.0mg/L)+NAA(0.1－0.4 mg/L).