Abstract:

Abstract: An endophytic fungus found from the leaves of Pyrethrum cinerariifolium Trev. , was identified as Fusarium sp. based on its morphological and biological characteristics. The bioassay results show that the inhibition rates of 10 time diluted fermentation liquid of this strain against 6 pathogenic fungi including Glomerella cingulata，Botrytis cirerea，Exserohilum turcicum， et al. ranged from 80.4％～93.3％, and that of the 5 time diluted fermentation liquid against B. cirerea、E. turcicum and G. cingulata were more than 65%. The effect of environmental and nutritional factors on its growth and reproduction was remarkable.ⅰ) The temperature from 4 ℃ to 40 ℃ were suitable for mycelial growth, sporulations and germination. The optimum temperature was 30 ℃ for sporulation, the lethal temperature was 60℃ for 10 minutes. ⅱ) pH value ranged from 4.0 to 9.5 was favourable for mycelial growth, sporulation and spore germination. The optimum pH was 7.5. ⅲ) As to nitrogen sources, the fungus utilized organic nitrogen very well. ⅳ) Taking D-xylose, sorbitol or mannitol as carbon source, with glycine, ammonium sulfate, beef extract or peptone as nitrogen source, the antifungal activity of the fungal fermenting liquid was comparatively high.